We determined the first molecular structure of a flagellar motor with a bound regulatory protein inside a living Caulobacter crescentus cell. With a new low-input “easy Hi-C” protocol, they mapped chromatin interactions in neural samples, defined cell-type-specific enhancer loops and aggregates, and concluded that Hi-C outperforms eQTL in explaining GWAS results. In addition, Micro-C provides a detailed map of nucleosome positions and localizes contact domain boundaries with nucleosomal precision. For all macro insights from Micro-C, see the protocol description in Molecular Cell, March 2020, and an exploration of the protocol’s full potential in Molecular Cell, March 2020. Compared to Hi-C, Micro-C exhibits an order of magnitude greater dynamic range, allowing the identification of ∼20,000 additional loops in each cell … In our opinion, Micro-4C, Micro-5C, or Micro-ChIP could be powerful alternatives for studies of single-locus or protein-centric connectome in ultra-high resolution. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally interacting domains and … Author information: (1)Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA … Our findings will help us to understand how nanomachines can be controlled at a molecular level. 506–521) describe long DNA methylation “canyons” that form very long-range, three-dimensional genomic interactions, specifically in hematopoietic stem cells.The cover depicts a landscape of hematopoietic differentiation. On the cover: In this issue of Molecular Cell, Zhang et al. 4) The Micro-C protocol is readily compatible with low-input cell numbers, as 1k–10k sorted cells have been successfully used to produce high-quality Micro-C maps.
We also discovered intriguing new features of the flagellar motor from this bacterium with unknown roles. (pp. However, don’t forget all about Hi-C yet; see what single-cell methods can do for you at Nature, March 2020.
Molecular biology / m ə ˈ l ɛ k j ʊ l ər / is the branch of biology that concerns the molecular basis of biological activity in and between cells, including molecular synthesis, modification, mechanisms and interactions.
Mapping Nucleosome Resolution Chromosome Folding in Yeast by Micro-C. Hsieh TH(1), Weiner A(2), Lajoie B(3), Dekker J(3), Friedman N(2), Rando OJ(4). DNA methylation is represented as mountains, while the glacier represents DNA methylation nadirs (canyons).